Cryo-Electron Tomography (cryo-ET)

Cellular electron cryotomography allows the study of individual microbial cells in their native state and in three dimensions at macromolecular resolutions. Our experiments rely on highly sophisticated and specialised equipment.

We have access to the electron microscopes at the NeCEN, the dutch cryo-electron microscopy center located in the Biology Institute of the Leiden University. The available instruments include a Talos L120C, Aquilos cryo-FIB, 2 TITAN Krios microscopes with state-of-the-art equipment for highest quality data collection, as well as fully equipped sample preparation laboratories, including  Leica instruments for sample freezing, large volume preprocessing and correlative imaging: cryo-ultramicrotome, high-pressure freezer (ICE), Thunder light microscope with cryostage and a cryo-plunger.